| Registro completo |
| Provedor de dados: |
Braz. J. Vet. Res. Anim. Sci.
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| País: |
Brazil
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| Título: |
Endocrine profiles and ovulation rate of cows superovulated with FSH following passive immunization against steroid free-bovine follicular fluid
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| Autores: |
ALVAREZ,Rafael Herrera
CARVALHO,João Batista Pereira de
ROSA E SILVA,Alzira
PERONE,Cibele Nunes
RIBELA,Maria Tereza Carvalho Pinto
OLIVEIRA FILHO,Enoch Borges de
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| Data: |
1998-01-01
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| Ano: |
1998
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| Palavras-chave: |
Superovulation
Follicular fluid
Passive immunization
Embryos
Cattle
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| Resumo: |
Ten multiparous non-lactating cows were randomly assigned to one of two groups. On days 8 to 12 after estrus, the first group was given 100 ml antiserum against steroid-free bovine follicular fluid (anti-bFF), which was produced in ovariectomized sheep. The second group (control) was given 100 ml nonimmune ovariectomized sheep serum. Six hours after injection, both groups were superovulated with FSH (18 NIH-FSH-S1 units) and LH (0.29 NIH-LH-S1 units) in a 4-day decreasing regime. On the morning of the third day a luteolytic dose of prostaglandin F2alpha analogue (cloprostenol) was given. Artificial inseminations (AI) were done 48 and 60 h later. Embryos were collected 7 days after AI by a nonsurgical method. Blood samples were taken throughout the experimental period and plasma concentrations of FSH, LH and progesterone were determined by radioimmunoassay. All cows from the immunized group, and 3 out of 5 cows in the control group, had more than 2 CL. There was no significant difference (P>0.05) in the ovulation rate between immunized and control groups (14.4 and 9.9, respectively). The number of recovered embryos was not significantly different (P>0.05) between groups, although cows immunized against bFF had a greater number of transferable embryos (3.4 ± 1.0 versus 0.8 ± 0.4, P<0.05). Plasma gonadotrophin concentrations were not correlated with either ovulation rate or number of recovered embryos. Plasma progesterone concentration was positively correlated (r = 0.88, P<0.01) to ovulation rate. Results suggested that passive immunization of cows with anti-bFF before superovulation did not reduce the variability of ovarian response.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961998000600007
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| Editor: |
Faculdade de Medicina Veterinária e Zootecnia / Universidade de São Paulo
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| Relação: |
10.1590/S1413-95961998000600007
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Veterinary Research and Animal Science v.35 n.6 1998
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| Direitos: |
info:eu-repo/semantics/openAccess
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